UK SBS PR16194 Side Entry Cold Stage for EM

The LMB (Laboratory of Molecular Biology-MRC Cambridge) wish to purchase single-tilt holder for cryo transfer and tomography of thin-film frozen-hydrated/vitrified specimens for use in the LMBs existing range of transmission electron microscopes (TEMs) with the following specifications: • The construction of the standard specimen cup/ holder tip material should be constructed from Beryllium copper material • Should be able to maintain a temperature below -175 C for 4 hours or more in Cryo Work Station or a TEM. • Time to reach minimum operating temperature 30 mins or less • Should be able to tilt to +/- 75 degrees • Should achieve a resolution at 0 degrees tilt of 0.34nm or higher • Should enable the frost-free transfer of cryo-specimens to the TEM stage • Should allow the user to effortlessly secure a grid without the use of additional detachable C-clips or clip-rings that can get lost or damaged. • Should allow tool-free specimen mounting • Should accept a 3 mm diameter TEM sample grid • Should have a low drift-rate of 1.5 nm/minute or better • The level of liquid nitrogen in the holder dewar should not affect the drift-rate of the holder so as automated data acquisition can be carried out without supervision for at least 4 hours or more. The closing date for this tender is the 14th September 2016 at 14:00 Additional information: The section below will provide instructions on how to submit a bid for this procurement The instructions are as follows; UK Shared Business Services eSourcing as part of the strategic alliance between UK Shared Business Services Ltd (UK SBS) and Crown Commercial Service (CCS), UK SBS can use the CCS eSourcing Suite for future procurements.

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